Co-operational PCR coupled with dot blot hybridization for detection and 16SrX grouping of phytoplasmas
نویسندگان
چکیده
منابع مشابه
Comparison of microplate hybridization with gel electrophoresis and dot blot hybridization for the rapid detection of Mycobacterium tuberculosis PCR products.
A microplate ELISA hybridization assay has been developed for the detection of the IS6110 PCR products of M. tuberculosis from sputum specimens. In this study, its efficacy was evaluated by comparison with agarose gel electrophoresis (AGE) and dot blot hybridization (DBH), with culture results as the 'gold standard'. The assay was used with 190 sputum samples: the PCR results detected by ELISA ...
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Transcriptase reverse polymerase chain reaction (RTPCR) and dot blot hybridization with digoxigenin-labeled probes were applied for the universal detection of Tospovirus species. The virus species tested were Tomato spotted wilt virus, Tomato chlorotic spot virus, Groundnut ringspot virus, Chrysanthemum stem necrosis virus, Impatiens necrotic spot virus, Zucchini lethal chlorosis virus, Iris ye...
متن کاملDevelopment of a recombinant protein-based dot-blot hybridization assay for the detection of antibody to chicken infectious bronchitis virus
Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, inducesstrong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of IBV antibody.Initially, 72 serum samples were tested for the presence of IBV antibody using a c...
متن کاملPCR-dot blot hybridization based on the neuraminidase-encoding gene is useful for detection of Bacteroides fragilis.
Bacteroides fragilis is a Gram-negative obligate anaerobe frequently isolated from clinical specimens and sometimes causes severe septicemia in compromised hosts. Increasing interest has been shown in the enterotoxigenicity and drug resistance of B. fragilis in the field of medical microbiology. We previously reported rapid detection of this anaerobe by nested PCR targeting a neuraminidase-enco...
متن کاملDetection of enterotoxigenic Escherichia coli by dot blot hybridization with biotinylated DNA probes.
A dot blot hybridization test was developed for the detection of enterotoxigenic E. coli without the use of radioisotopes. Three biotin-labeled DNA (Bio-DNA) probes corresponding to structural genes specifying heat-labile and heat-stable enterotoxins of porcine and human origin were prepared by random priming; label incorporation was significantly higher than that obtained from the use of nick ...
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ژورنال
عنوان ژورنال: Plant Pathology
سال: 2007
ISSN: 0032-0862,1365-3059
DOI: 10.1111/j.1365-3059.2007.01601.x